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Rohem Improved Neubauer Counting Chamber – Silverlite

1,149.00pc

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A precision-milled glass counting chamber designed for quantitative cell counting under a microscope. The Rohem Silverlite Improved Neubauer model uses a Brightline grid with two counting areas (cells) and is ideal for counting red blood cells (RBC), white blood cells (WBC), platelets, sperm or other microscopic particles in a known volume. Its optically polished glass construction ensures accurate, consistent results in clinical, research, pathology, or teaching labs.

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Description

Specifications:

  • Improved Neubauer grid: Precisely engraved grid with standard 9 large squares and a subdivided central area for detailed counting (25 × 16 small squares) according to improved Neubauer design.

  • Dual counting cells: Two independent chambers allow counting in duplicate to improve accuracy and repeatability.

  • Brightline contrast: Grid lines appear sharp and bright under standard microscope illumination (enhances visibility and reduces counting errors).

  • Optically polished glass: High-quality glass construction with polished surfaces for clear microscopy.

  • Standard depth: Chamber depth of 0.1 mm (1/10 mm), giving a known volume over each square — essential for calculating concentration per unit volume.

  • Easy loading: Fluid fills the counting chamber by capillary action under the specially matched coverslip.

Applications:

  • Hematology & clinical labs: Manual counting of RBCs, WBCs, differential cell counts, and platelets.

  • Microbiology & cell culture: Estimating concentration of cultured cells, yeast, bacteria with appropriate staining.

  • Semen analysis: Counting sperm concentration in fertility testing workflows.

  • Environmental & research labs: Quantifying particulate matter, microorganisms, or cells in environmental samples.

  • Teaching & training labs: Demonstrating manual counting techniques in educational settings.

Technicals:

Parameter

SPECIFICATION

Brand

Rohem Instruments

Model

Improved Neubauer Counting Chamber – Silverlite

Counting Grid

Improved Neubauer grid with dual cells

Grid Structure

9 large squares with subdivided central area (400 small squares)

Chamber Depth

0.10 mm (1/10 mm)

Volume per Square mm

~0.0001 µL (0.1 mm depth × 1 mm² area)

Counting Cells

2 (two independent chambers)

Precations:

  • Handle with care — glass is fragile and can break if dropped or knocked.

  • Clean thoroughly before and after use with appropriate solutions (e.g., 70 % ethanol) to avoid contamination.

  • Ensure coverslip sits evenly and the chamber is free of bubbles before loading sample to avoid volume errors.

  • Use in conjunction with appropriate microscope magnification and counting protocols (e.g., Wright/Giemsa staining for blood).

FAQ:

Q: What is a counting chamber used for?
A: A counting chamber (hemocytometer) is a precision glass slide with an engraved grid used to count cells or particles in a defined volume, allowing calculation of concentration.

Q: How does the improved Neubauer grid help?
A: The improved grid provides a standard pattern that makes counting easier and more accurate by subdividing areas into known volumes and offering contrast lines for clear visibility.

Q: Why are there two chambers?
A: Dual chambers allow replicate counts (e.g., count both sides) to increase accuracy and reduce sampling error.

Q: What do I need to use this chamber?
A: You need a compatible hemocytometer coverslip, microscope (usually 10× objective for counting grids), and sample suspension prepared appropriately.

Disclaimer

Please note that the product images displayed on our website may exhibit variations in color, markings etc as per the availability in stock. We recommend contacting us via WhatsApp or email for confirmation before placing your order.

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